Open AccessTranscription of cloned transfer RNA genes fromDrosophila melanogasterin a homologous cell-free extract
Author(s) -
Bhanu Rajput,
Loverne Duncan,
Dianne DeMille,
Robert C. Miller,
George B. Spiegelman
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.20.6541
Subject(s) - miller , george (robot) , biology , library science , columbia university , drosophila melanogaster , history , genetics , gene , art history , media studies , sociology , ecology , computer science
Cloned Drosophila melanogaster tRNA genes have been transcribed in a homologous cell-free extract isolated from a Schneider II cell line. The major product of the reaction is a tRNA precursor which is processed to a tRNA sized species. The kinetics of transcription has been followed for 5 different valine tRNA gene clones. The results demonstrate formation of stable transcription complex with at least two kinetic steps. While the rate of formation of the transcription complex is similar to different clones, the ultimate rate of transcription varies dramatically. Comparison of the DNA sequence of the tRNA genes suggests that rate determining nucleotides lie outside the canonical tRNA split-internal promoters.