A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of λ cro binding heptadecanucleotide on a polymer support | Zendy

Eiko Ohtsuka | Zendy; Zenzaburo Tozuka | Zendy; Shigenori Iwai | Zendy; Morio Ikehara | Zendy

Open Access

A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of λ cro binding heptadecanucleotide on a polymer support

Author(s) -

Eiko Ohtsuka,

Zenzaburo Tozuka,

Shigenori Iwai,

Morio Ikehara

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.20.6235

Subject(s) - biology , reagent , polymer , combinatorial chemistry , biochemistry , computational biology , organic chemistry , chemistry

A new condensing reagent 1-(2,4,6,-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide (TPSPy) was found to give one diastereoisomer of dinucleoside monophosphate aryl esters. Several oligodeoxynucleotide blocks were prepared using this reagent. A heptadecanucleotide, dTATCCCTTGCGGTGATA, which had the same sequence as the lambda cro binding DNA sequence was synthesized by condensing mono-, tri- and dodecanucleotide blocks using this reagent on a polystyrene support.

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