Open AccessModified porynudeotides. VI. Properties of a synthetic DNA containing the anti-herpes agent (E)-5-(2-bromovinyI)-2′-deoxyuridine
Author(s) -
János Sági,
A.B. Czuppon,
M. Kajtár,
Anna Szabolcs,
Attila Szemzö,
László Ötvös
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.19.6051
Subject(s) - deoxyuridine , circular dichroism , biology , dna , thermal stability , deoxyadenosine , polymerase , dna polymerase , nucleotide , bioorganic chemistry , biochemistry , enzyme , deoxyribonucleoside , stereochemistry , combinatorial chemistry , chemistry , organic chemistry , gene
A new modified polydeoxynucleotide, a copolymer of nucleotides of 2'-deoxyadenosine and the very efficacious anti-herpesvirus agent (E)-5-(2-bromovinyl)-2'-deoxyuridine was synthesized with E. coli DNA polymerase I enzyme. It is characterized by its physical (absorption and circular dichroism spectra, thermal transition, sedimentation analysis) and bioorganic (template activity, stability) properties. Compared to poly [d(A-T)], the modified polydeoxynucleotide had a lower thermal stability but exhibited higher stability against DNases and higher template activity for DNA synthesis. Template activity for RNA synthesis of this template was, however, poor and extent of AMP and UMP incorporation was limited as well.