Open AccessNew restriction endonucleases fromAcetobacter acetiandBacillus aneurinolyticus
Author(s) -
Hiroyuki Sugisaki,
Yoshihiko Maekawa,
Susumu Kanazawa,
Mituru Takanami
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.19.5747
Subject(s) - biology , restriction enzyme , recognition sequence , acetobacter , genetics , bacillus (shape) , restriction map , cleavage (geology) , restriction fragment , bacteria , dna , base sequence , paleontology , fracture (geology)
Two restriction endonucleases with new sequence specificities have been isolated from Acetobacter aceti IFO 3281 and Bacillus aneurinolyticus IAM 1077 and named AatII and BanII, respectively. Based on analysis of the sequences around the restriction sites, the recognition sequences and cleavage sites of these endonucleases were deduced as below: (formula; see text)