Open Access5S RNA sequence from thePhilosamia silkworm: evidence for variable evolutionary rates in insect 5S RNA
Author(s) -
Gu Xian-Rong,
Krikor Nicoghosian,
Robert Cedergren
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.18.5711
Subject(s) - biology , rna , bombyx mori , genetics , sequence (biology) , nucleic acid secondary structure , bombyx , phylogenetic tree , drosophila melanogaster , conserved sequence , nucleotide , nucleic acid structure , evolutionary biology , dna , base sequence , gene
The primary structure of 5S RNA isolated from the posterior silkgland of Philosamia cynthia ricini was determined using three in vitro labelling techniques. The derived sequence consists of 119 nucleotides and can be folded into the secondary structure model proposed for eukaryotic 5S RNAs. This 5S RNA differs from the Bombyx mori molecule in 9 positions and from the Drosophila melanogaster sequence in 14 positions. The comparison of evolutionary rates in insect 5S RNA with inferred rates in other eukaryotic phyla leads to the conclusion that 5S RNA evolution is not constant in different eukaryotic branches, a condition which must be taken into account in phylogenetic tree constructions.