Open AccessEpsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos
Author(s) -
Maggi Alan,
Guillermo Grindlay,
Lorraine Stefani,
John Paul
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.17.5133
Subject(s) - biology , primer extension , microbiology and biotechnology , gene , messenger rna , globin , primer (cosmetics) , coding region , rna , transcription (linguistics) , embryo , genetics , nucleotide , linguistics , chemistry , philosophy , organic chemistry
RNA transcribed from the human epsilon globin gene was studied in K562 cells and human embryos of 5-10 weeks gestation. Using both primer extension and S1 analysis RNA molecules were found which extend 53 +/- 1 bp, 55 +/- 1 bp and 270 +/- 1 bp upstream from the first coding base and are colinear with the gene. The first pair of molecules represent transcripts initiating at the canonical cap site but the other species represents a transcript over 200 nucleotides longer which initiates upstream of the CAAT and ATAA boxes.