Open AccessSequence of the intercistronic region between the ribulose-1,5-bishphosphate carboxylase/oxgenase large subunit and the coupling factor β subunit gene
Author(s) -
Kazuo Shinozaki,
Masahiro Sugiura
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.16.4923
Subject(s) - biology , specificity factor , chloroplast dna , protein subunit , nucleic acid sequence , gene , microbiology and biotechnology , genetics , terminator (solar) , rna polymerase , rna , chloroplast , ionosphere , physics , astronomy
The nucleotide sequence of the region between the genes for the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase and the beta subunit of coupling factor from tobacco chloroplast DNA has been determined. The 5' ends of their mRNAs have been located on the DNA sequence using s1 nuclease mapping. The promoter sequences of these chloroplast genes have features in common with those of prokaryotes. E. coli RNA polymerase can recognize these sequences and start transcription at authentic initiation sites.