Open AccessBlotting of RNA onto ion exchange paper allowing subsequent characterization byin situtranslation in addition to blot hybridization
Author(s) -
Christiaan J. M. Saris,
Henk Franssen,
Jos H. Heuyerjans,
Jet van Eenbergen,
H. P. J. Bloemers
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.16.4831
Subject(s) - biology , rna , blot , in situ hybridization , northern blot , microbiology and biotechnology , transfer rna , dot blot , nucleic acid thermodynamics , translation (biology) , southern blot , messenger rna , biochemistry , dna , gene
We present the preparation of an ion exchange paper which can be used as a solid carrier in the transfer of RNA from gels. In addition to detection by blot hybridization to specific probes, transferred RNA can be characterized by cell-free translation in situ. Preparation of the paper is simple, inexpensive and reproducible. Examples of applications are shown and possible other applications are discussed.