Blotting of RNA onto ion exchange paper allowing subsequent characterization byin situtranslation in addition to blot hybridization | Zendy

Chris Saris | Zendy; Henk Franssen | Zendy; Jos H. Heuyerjans | Zendy; Jet van Eenbergen | Zendy; Henri P.J. Bloemers | Zendy

Open Access

Blotting of RNA onto ion exchange paper allowing subsequent characterization byin situtranslation in addition to blot hybridization

Author(s) -

Chris Saris,

Henk Franssen,

Jos H. Heuyerjans,

Jet van Eenbergen,

Henri P.J. Bloemers

Publication year - 1982

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/10.16.4831

Subject(s) - biology , rna , blot , in situ hybridization , northern blot , microbiology and biotechnology , transfer rna , dot blot , nucleic acid thermodynamics , translation (biology) , southern blot , messenger rna , biochemistry , dna , gene

We present the preparation of an ion exchange paper which can be used as a solid carrier in the transfer of RNA from gels. In addition to detection by blot hybridization to specific probes, transferred RNA can be characterized by cell-free translation in situ. Preparation of the paper is simple, inexpensive and reproducible. Examples of applications are shown and possible other applications are discussed.

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