Open AccessSequence-specific cleavage of chromatin by staphylococcal nuclease can generate an atypical nuclesome pattern
Author(s) -
Urs Pauli,
Thomas Seebeck,
Richard Braun
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.14.4121
Subject(s) - biology , cleavage (geology) , chromatin , nuclease , library science , classics , dna , genetics , history , computer science , paleontology , fracture (geology)
We have investigated the nucleosomal organization of ribosomal genes in the acellular slime mold Physarum polycephalum. When probed with staphylococcal nuclease, the ribosomal genes appear to be uniformly packed in nucleosomes, in an arrangement which is indistinguishable from the pattern obtained with bulk chromatin. During this study, an unusual pattern of digestion was obtained from a DNA region immediately upstream of the initiation site of rRNA transcription, in addition to the nucleosomal profile, a second regular ladder of fragments with a repeat length of 30-40 basepairs was generated from this region. We established that this pattern of degradation reflects the strong preference of staphylococcal nuclease for certain nucleotide arrangements on the DNA, rather than a particular chromatin configuration. These observations clearly show that great caution needs to be exerted whenever data from staphylococcal nuclease digestions are interpreted in terms of chromatin structure.