Open AccessComparison of the nucleotide sequence of cloned human and guinea-pig pre-α-lactalbumin cDNA with that of chick pre-lysozyme cDNA suggests evolution from a common ancestral gene
Author(s) -
Len Hall,
Roger K. Craig,
Mark R. Edbrooke,
P. N. Campbell
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.11.3503
Subject(s) - biology , complementary dna , nucleic acid sequence , gene , lactalbumin , genetics , lysozyme , microbiology and biotechnology , biochemistry
Nucleotide sequence analyses of essentially full-length copies of human and guinea-pig pre-alpha-lactalbumin cDNAs contained within recombinant plasmids, (i) confirm the presence of 19 amino acid hydrophobic amino terminal peptide extensions encoded within each mRNA; and (ii) provides evidence for the existence of a minor variant of guinea-pig alpha-lactalbumin mRNA encoding a protein with a 36 residue carboxyl-terminal extension. Comparison of the nucleotide sequence within the coding region of the human, and the predominant guinea-pig pre-alpha-lactalbumin mRNAs, with the analogous region of hen pre-lysozyme mRNA provides compelling evidence that all have evolved from a common ancestral gene.