Open AccessChromatin diminution inAscaris suum: nucleotide sequence of the eliminated satellite DNA
Author(s) -
Rolf E. Streeck,
Karl B. Moritz,
Katrin Beer
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.11.3495
Subject(s) - biology , ascaris suum , chromatin , satellite dna , dna , nucleic acid sequence , genetics , ascaris , satellite , sequence (biology) , nucleotide , dna sequencing , microbiology and biotechnology , gene , genome , zoology , helminths , engineering , aerospace engineering
We have determined the prototype sequence of the DNA which is eliminated in the course of chromatin diminution in Ascaris suum. This DNA which is virtually absent from somatic cells but retained in the germ line consists predominantly of highly repetitive sequences which are variants of an AT rich 123 base pair repeat unit. Both major and minor variants have been sequenced. The overall structure of this germ line limited DNA corresponds to the segmental organization characteristic of satellite DNAs. Possible correlations between the mechanism of chromatin diminution and some properties of the satellite sequence are discussed.