Open AccessThe anticodon of the maize chloroplast gene for tRNAUAALeuis split by a large intron
Author(s) -
André Steinmetz,
Earl J. Gubbins,
Lawrence Bogorad
Publication year - 1982
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/10.10.3027
Subject(s) - transfer rna , biology , intron , gene , chloroplast , rna splicing , genetics , chloroplast dna , base pair , nucleotide , nucleic acid sequence , rna
The maize chloroplast gene encoding tRNA Leu UAA has been sequenced. It contains a 458 base pair intron between the first and second bases of the anticodon. The tRNA is 88 nucleotides long (the 3'-terminal CCA sequence included which, however, is not encoded by the gene) and differs in only four nucleotides (modified nucleotides are not considered) from the corresponding isoacceptor from bean chloroplasts. The unusual position of the intron in this maize chloroplast tRNA gene suggests a splicing model different from that generally accepted for eukaryotic split tRNA genes.