Open AccessCulturing Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) for Diagnosis and Genome Sequencing
Author(s) -
Zhiqi Zeng,
Hua Guo,
Liping Chen,
Zhengshi Lin,
Weijun Guan,
Yutao Wang,
Haiming Jiang,
Xihan Wu,
Yong Yin,
Zelong Gao,
Canxiong Chen,
Zifeng Yang
Publication year - 2022
Publication title -
laboratory medicine
Language(s) - English
Resource type - Journals
eISSN - 1943-7730
pISSN - 0007-5027
DOI - 10.1093/labmed/lmac060
Subject(s) - viral load , virology , biology , virus , polymerase chain reaction , viral culture , nucleic acid test , nucleic acid , real time polymerase chain reaction , coronavirus , metagenomics , gene , covid-19 , medicine , infectious disease (medical specialty) , genetics , disease , pathology
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid detection "re-positive" phenomenon is encountered clinically. The accuracy of a viral nucleic acid test is crucial to prevent reintroduction of the virus into the community. This study evaluated the effect of virus culturing on increasing the sensitivity and specificity of real-time polymerase chain reaction (RT-PCR) detection and viral genomic sequencing.