Culturing Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) for Diagnosis and Genome Sequencing | Zendy

Zhiqi Zeng | Zendy; Hua Guo | Zendy; Liping Chen | Zendy; Zhengshi Lin | Zendy; Wenda Guan | Zendy; Yutao Wang | Zendy; Haiming Jiang | Zendy; Xiao Wu | Zendy; Yong Yin | Zendy; Zelong Gao | Zendy; Canxiong Chen | Zendy; Zifeng Yang | Zendy

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Culturing Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) for Diagnosis and Genome Sequencing

Author(s) -

Zhiqi Zeng,

Hua Guo,

Liping Chen,

Zhengshi Lin,

Wenda Guan,

Yutao Wang,

Haiming Jiang,

Xiao Wu,

Yong Yin,

Zelong Gao,

Canxiong Chen,

Zifeng Yang

Publication year - 2022

Publication title -

laboratory medicine

Language(s) - English

Resource type - Journals

eISSN - 1943-7730

pISSN - 0007-5027

DOI - 10.1093/labmed/lmac060

Subject(s) - viral load , virology , biology , virus , polymerase chain reaction , viral culture , nucleic acid test , nucleic acid , real time polymerase chain reaction , coronavirus , metagenomics , gene , covid-19 , medicine , infectious disease (medical specialty) , genetics , disease , pathology

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid detection "re-positive" phenomenon is encountered clinically. The accuracy of a viral nucleic acid test is crucial to prevent reintroduction of the virus into the community. This study evaluated the effect of virus culturing on increasing the sensitivity and specificity of real-time polymerase chain reaction (RT-PCR) detection and viral genomic sequencing.

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