RNF219 interacts with CCR4–NOT in regulating stem cell differentiation | Zendy

Hao Du | Zendy; Chen Chen | Zendy; Yan Wang | Zendy; Yang Yang | Zendy; Zhuanzhuan Che | Zendy; Xiaoxu Liu | Zendy; Siyan Meng | Zendy; Chenghao Guo | Zendy; Manman Xu | Zendy; Haitong Fang | Zendy; Fengchao Wang | Zendy; Chengqi Lin | Zendy; Zhuojuan Luo | Zendy

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RNF219 interacts with CCR4–NOT in regulating stem cell differentiation

Author(s) -

Hao Du,

Chen Chen,

Yan Wang,

Yang Yang,

Zhuanzhuan Che,

Xiaoxu Liu,

Siyan Meng,

Chenghao Guo,

Manman Xu,

Haitong Fang,

Fengchao Wang,

Chengqi Lin,

Zhuojuan Luo

Publication year - 2020

Publication title -

journal of molecular cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.825

H-Index - 62

eISSN - 1674-2788

pISSN - 1759-4685

DOI - 10.1093/jmcb/mjaa061

Subject(s) - stem cell , microbiology and biotechnology , cellular differentiation , biology , computational biology , genetics , gene

Regulation of RNA stability plays a crucial role in gene expression control. Deadenylation is the initial rate-limiting step for the majority of RNA decay events. Here, we show that RING finger protein 219 (RNF219) interacts with the CCR4-NOT deadenylase complex. RNF219-CCR4-NOT exhibits deadenylation activity in vitro. RNA-seq analyses identify some of the 2-cell-specific genes and the neuronal genes significantly downregulated upon RNF219 knockdown, while upregulated after depletion of the CCR4-NOT subunit CNOT10 in mouse embryonic stem (ES) cells. RNF219 depletion leads to impaired neuronal lineage commitment during ES cell differentiation. Our study suggests that RNF219 is a novel interacting partner of CCR4-NOT and required for maintenance of ES cell pluripotency.

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