Open AccessCharacterization of cross-species transcription and splicing from Penicillium to Saccharomyces cerevisiae
Author(s) -
Zhenquan Lin,
Kang Xu,
Cai Guang,
Yangqingxue Liu,
Yang Li,
Zhihao Zhang,
Jens Nielsen,
Shuobo Shi,
Zihe Liu
Publication year - 2021
Publication title -
journal of industrial microbiology and biotechnology/journal of industrial microbiology and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1476-5535
pISSN - 1367-5435
DOI - 10.1093/jimb/kuab054
Subject(s) - saccharomyces cerevisiae , biology , ura3 , rna splicing , yeast , aspergillus nidulans , genetics , transcription (linguistics) , promoter , gene , intron , penicillium , heterologous expression , kluyveromyces lactis , gene expression , rna , mutant , linguistics , philosophy , recombinant dna
Heterologous expression of eukaryotic gene clusters in yeast has been widely used for producing high-value chemicals and bioactive secondary metabolites. However, eukaryotic transcription cis-elements are still undercharacterized, and the cross-species expression mechanism remains poorly understood. Here we used the whole expression unit (including original promoter, terminator, and open reading frame with introns) of orotidine 5′-monophosphate decarboxylases from 14 Penicillium species as a showcase, and analyzed their cross-species expression in Saccharomyces cerevisiae. We found that pyrG promoters from the Penicillium species could drive URA3 expression in yeast, and that inefficient cross-species splicing of Penicillium introns might result in weak cross-species expression. Thus, this study demonstrates cross-species expression from Penicillium to yeast, and sheds light on the opportunities and challenges of cross-species expression of fungi expression units and gene clusters in yeast without refactoring for novel natural product discovery.