Polymyxin resistance in Klebsiella pneumoniae: multifaceted mechanisms utilized in the presence and absence of the plasmid-encoded phosphoethanolamine transferase gene mcr-1 | Zendy

Sue C. Nang | Zendy; MeiLing Han | Zendy; Heidi H. Yu | Zendy; Jiping Wang | Zendy; Von Vergel L Torres | Zendy; Chongshan Dai | Zendy; Tony Velkov | Zendy; Marina Harper | Zendy; Jian Li | Zendy

Open Access

Polymyxin resistance in Klebsiella pneumoniae: multifaceted mechanisms utilized in the presence and absence of the plasmid-encoded phosphoethanolamine transferase gene mcr-1

Author(s) -

Sue C. Nang,

MeiLing Han,

Heidi H. Yu,

Jiping Wang,

Von Vergel L Torres,

Chongshan Dai,

Tony Velkov,

Marina Harper,

Jian Li

Publication year - 2019

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkz314

Subject(s) - klebsiella pneumoniae , polymyxin , biology , polymyxin b , microbiology and biotechnology , lipid a , antibiotics , bacteria , gene , escherichia coli , biochemistry , genetics

Until plasmid-mediated mcr-1 was discovered, it was believed that polymyxin resistance in Gram-negative bacteria was mainly mediated by the chromosomally-encoded EptA and ArnT, which modify lipid A with phosphoethanolamine (pEtN) and 4-amino-4-deoxy-l-arabinose (l-Ara4N), respectively. This study aimed to construct a markerless mcr-1 deletion mutant in Klebsiella pneumoniae, validate a reliable reference gene for reverse transcription quantitative PCR (RT-qPCR) and investigate the interactions among mcr-1, arnT and eptA, in response to polymyxin treatments using pharmacokinetics/pharmacodynamics (PK/PD).

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research