Open AccessAttenuation of Helper T Cell Capacity for TH1 and TH17 Differentiation in Children With Nontuberculous Mycobacterial Infection
Author(s) -
Tiffany A. Claeys,
Oscar Rosas Mejia,
Samuel F. Marshall,
Jason A. Jarzembowski,
Don Hayes,
Natalie M. Hull,
Namal P. M. Liyanage,
Robert H. Chun,
Cecille G. Sulman,
Anna R. Huppler,
Richard Robinson
Publication year - 2019
Publication title -
the journal of infectious diseases (online. university of chicago press)/the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1093/infdis/jiz371
Subject(s) - nontuberculous mycobacteria , immunology , lymph node , cytokine , interferon gamma , lymph , interleukin 17 , polyclonal antibodies , biology , cellular differentiation , medicine , mycobacterium , pathology , antigen , tuberculosis , biochemistry , gene
Nontuberculous mycobacteria (NTM) infect children with increasing frequency worldwide. Using blood and lymph node tissue from children with NTM lymphadenitis, and uninfected lymph node tissue from community controls, we evaluated helper T (TH) cells in functional assays of TH1/TH17 differentiation and measured the concentration of their associated cytokines at the site of infection. Circulating TH cells from infected children were attenuated in their TH1/TH17 differentiation capacity and expressed less interferon γ and interleukin 17 after polyclonal stimulation. Similar differences were observed at the site of infection, where most cytokine concentrations were unchanged relative to controls. Our data are consistent with a model wherein TH1/TH17 differentiation is attenuated in NTM-infected children.