Open AccessLongitudinal Dynamics of Intact HIV Proviral DNA and Outgrowth Virus Frequencies in a Cohort of Individuals Receiving Antiretroviral Therapy
Author(s) -
Shane D. Falcinelli,
Kayla W. Kilpatrick,
Jenna Read,
Ross Murtagh,
Brigitte Allard,
Simon Ghofrani,
Jennifer Kirchherr,
Katherine S James,
Erin Stuelke,
Caroline Baker,
JoAnn Kuruc,
Joseph J. Eron,
Michael G. Hudgens,
Cynthia L. Gay,
David M. Margolis,
Nancie M. Archin
Publication year - 2020
Publication title -
the journal of infectious diseases (online. university of chicago press)/the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1093/infdis/jiaa718
Subject(s) - provirus , virology , viral replication , virus , biology , replication (statistics) , dna replication , antiretroviral therapy , human immunodeficiency virus (hiv) , dna , viral quasispecies , viral load , immunology , genetics , genome , gene , hepatitis c virus
The replication-competent human immunodeficiency virus (HIV) reservoir is the major barrier to cure. The quantitative viral outgrowth assay (QVOA), the gold-standard method to quantify replication-competent HIV, is resource intensive, which limits its application in large clinical trials. The intact proviral DNA assay (IPDA) requires minimal cell input relative to QVOA and quantifies both defective and intact proviral HIV DNA, the latter potentially serving as a surrogate marker for replication-competent provirus. However, there are limited cross-sectional and longitudinal data on the relationship between IPDA and QVOA measurements.