z-logo
open-access-imgOpen Access
DgMYB2 improves cold resistance in chrysanthemum by directly targeting DgGPX1
Author(s) -
Xiaohan Yang,
Yunchen Luo,
Huiru Bai,
Xin Li,
Si Tang,
Xiaoqin Liao,
Lei Zhang,
Qinglin Liu
Publication year - 2022
Publication title -
horticulture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.947
H-Index - 31
eISSN - 2662-6810
pISSN - 2052-7276
DOI - 10.1093/hr/uhab028
Subject(s) - biology , chrysanthemum morifolium , transcription factor , microbiology and biotechnology , myb , chromatin , cold tolerance , cold stress , gene , genetics , botany
MYB transcription factors play important roles in plant responses to cold stress, but the associated underlying mechanisms remain unclear. In this study, a cold-induced MYB transcription factor, DgMYB2, was isolated from chrysanthemum (Chrysanthemum morifolium Ramat). DgMYB2 was localized to the nucleus and exhibited transactivational activity. Overexpression of DgMYB2 improved cold tolerance in chrysanthemum, while cold tolerance in the antisense suppression lines decreased compared to that of the wild type. Additionally, electrophoretic mobility shift assays, chromatin immunoprecipitation, luciferase complementary imaging analysis, and dual-luciferase reporter gene detection experiments confirmed that DgMYB2 directly targets DgGPX1 and increases the activity of glutathione peroxidase to reduce the accumulation of reactive oxygen species, thereby improving cold resistance in chrysanthemum.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here
Accelerating Research

Address

John Eccles House
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom