Open AccessTHE STUDY OF GENIC VARIATION BY ELECTROPHORETIC AND HEAT DENATURATION TECHNIQUES AT THE OCTANOL DEHYDROGENASE LOCUS IN MEMBERS OF THE DROSOPHILA VIRILIS GROUP
Author(s) -
Rama S. Singh,
John L. Hubby,
Lynn H. Throckmorton
Publication year - 1975
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/80.3.637
Subject(s) - locus (genetics) , biology , drosophila virilis , allele , genetics , electrophoresis , microbiology and biotechnology , drosophila (subgenus) , gene
Heat denaturation studies of three different electrophoretic allozymes of octanol dehydrogenase were performed from 10 species of the Drosophila virilis group. A total of 18 alleles were discovered, and in 3 species in which electrophoretic studies indicated that the locus was monomorphic, heat denaturation showed that the locus was polymorphic. We show that electrophoretic studies underestimate the number of alleles at this locus by a factor of 2.6 in these species. The results are discussed in the light of the continuing controversy over selection and neutral theories of genetic variation.