Open AccessINDUCTION BY MUTAGENS OF TANDEM GENE DUPLICATIONS IN THE glyS REGION OF THE ESCHERICHIA COLI CHROMOSOME
Author(s) -
Daniel S. Straus
Publication year - 1974
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/78.3.823
Subject(s) - biology , escherichia coli , reversion , genetics , mutagenesis , mutant , mutagen , gene , chromosome , mutation , microbiology and biotechnology , mutation frequency , ultraviolet light , dna , chemistry , photochemistry , phenotype
Four mutagens (ultraviolet light, nitrous acid, the acridine half mustard ICR 372, and niridazole) have been found to increase the frequency of tandem gene duplications in the glyS region of the Escherichia coli chromosome. This result was obtained by quantitating the spontaneous and mutagen-induced reversion frequency of a glycyl-tRNA synthetase (glyS) mutant. Following mutagenesis, as many as 0.2% of the survivors were observed to contain duplications in the glyS region. In addition, several classes of stable revertants of the glyS mutant have been identified.