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Through use of tetrad, random spore, trisomic, and mitotic analysis procedures a large number of genes, including 48 new genetic markers, were studied for their locations on the genetic maps of the yeast Saccharomyces cerevisiae. Eighteen new centromere linked genes were discovered and all but one was located on various ones of the 16 previously-established chromosomes. Five fragments of linked genes were also assigned to chromosomes; four were located on known chromosomes while the fifth determined one arm of a new chromosome. The experiments indicate that seventeen is likely to be the haploid chromosome number in this yeast. Most chromosomes have been established by genetic means to be metacentric and their genetic lengths vary from 5 cM to approximately 400 cM. Functionally-related sets of genes generally were found to be dispersed over the genome.

GENETIC MAPPING IN SACCHAROMYCES IV. MAPPING OF TEMPERATURE-SENSITIVE GENES AND USE OF DISOMIC STRAINS IN LOCALIZING GENES