Open AccessIdentification of a Functional Domain Within the Essential Core of Histone H3 That Is Required for Telomeric and HM Silencing in Saccharomyces cerevisiae
Author(s) -
Jeffrey S. Thompson,
Marilyn L Snow,
Summer Giles,
Leslie McPherson,
Michael Grunstein
Publication year - 2003
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/163.1.447
Subject(s) - biology , derepression , genetics , saccharomyces cerevisiae , histone , heterochromatin protein 1 , histone h3 , heterochromatin , histone methylation , gene silencing , histone h2a , histone methyltransferase , psychological repression , chromatin , dna methylation , yeast , dna , gene , gene expression
Fourteen novel single-amino-acid substitution mutations in histone H3 that disrupt telomeric silencing in Saccharomyces cerevisiae were identified, 10 of which are clustered within the alpha1 helix and L1 loop of the essential histone fold. Several of these mutations cause derepression of silent mating locus HML, and an additional subset cause partial loss of basal repression at the GAL1 promoter. Our results identify a new domain within the essential core of histone H3 that is required for heterochromatin-mediated silencing.