Open AccessIdentification of 1088 New Transposon Insertions of Caenorhabditis elegans: A Pilot Study Toward Large-Scale Screens
Author(s) -
Edwige Martin,
Hélène Laloux,
Gaëlle Couette,
Thierry Alvarez,
Catherine Bessou,
Oliver Hauser,
Satis Sookhareea,
Michel Labouesse,
Laurent Ségalat
Publication year - 2002
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/162.1.521
Subject(s) - caenorhabditis elegans , transposable element , biology , genetics , mutant , phenotype , dna transposable elements , gene , identification (biology) , computational biology , botany
We explored the feasibility of a strategy based on transposons to generate identified mutants of most Caenorhabditis elegans genes. A total of 1088 random new insertions of C. elegans transposons Tc1, Tc3, and Tc5 were identified by anchored PCR, some of which result in a mutant phenotype.