Open AccessSSCP Analysis of cDNA Markers Provides a Dense Linkage Map of the Aedes aegypti Genome
Author(s) -
Ruth E. Fulton,
Michael L. Salasek,
Nancy M. DuTeau,
William C. Black
Publication year - 2001
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/158.2.715
Subject(s) - biology , genetics , aedes aegypti , synteny , single nucleotide polymorphism , genbank , single strand conformation polymorphism , genome , genetic linkage , primer (cosmetics) , population , gene , polymerase chain reaction , genotype , larva , chemistry , botany , demography , organic chemistry , sociology
An intensive linkage map of the yellow fever mosquito, Aedes aegypti, was constructed using single-strand conformation polymorphism (SSCP) analysis of cDNA markers to identify single nucleotide polymorphisms (SNPs). A total of 94 A. aegypti cDNAs were downloaded from GenBank and primers were designed to amplify fragments <500 bp in size. These primer pairs amplified 94 loci, 57 (61%) of which segregated in a single F(1) intercross family among 83 F(2) progeny. This allowed us to produce a dense linkage map of one marker every 2 cM distributed over a total length of 134 cM. Many A. aegypti cDNAs were highly similar to genes in the Drosophila melanogaster genome project. Comparative linkage analysis revealed areas of synteny between the two species. SNP polymorphisms are abundant in A. aegypti genes and should prove useful in both population genetics and mapping studies.