Open AccessLocalization of the nic-7, ac-29 and thi-10 genes within the mating-type locus of Chlamydomonas reinhardtii.
Author(s) -
Patrick J. Ferris
Publication year - 1995
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/141.2.543
Subject(s) - chlamydomonas reinhardtii , biology , locus (genetics) , genetics , gene , mating type , chlamydomonas , selectable marker , recombination , transformation (genetics) , mutant
The tight linkage observed between the mating-type (mt) locus of Chlamydomonas reinhardtii and three auxotrophic mutations--nic-7 (nicotinamide-requiring), ac-29 (acetate-requiring), and thi-10 (thiamine-requiring)--has led to the hypothesis that recombination is suppressed in the mt region. The physical location of these three genes has been established by transformation with sets of cloned DNA from the mt region. They lie to the left and right of the highly rearranged (R) domain of the mt locus, which has been proposed to be responsible for teh recombinational suppression in the region. The cloned nic-7+ and thi-10+ genes will be useful as selectable markers for cotransformation markers for cotransformation experiments.