Open AccessDisruption of a silencer domain by a retrotransposon.
Author(s) -
M F Mastrangelo,
Keith G. Weinstock,
Brenda K. Shafer,
Anne-Marie Hedge,
David Garfinkel,
Jeffrey N. Strathern
Publication year - 1992
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/131.3.519
Subject(s) - biology , retrotransposon , genetics , gene silencing , locus (genetics) , mutant , gene , transposable element , insertion , silencer , mechanical engineering , engineering , inlet
A galactose-inducible Ty element carrying the HIS3 gene has been used as an insertional mutagen to generate alpha-factor resistant mutants. This collection of Ty-induced mutations includes insertions into the gene for the alpha-factor receptor (STE2), several nonspecific STE genes, and mutations that lead to the expression of the normally silent HML alpha locus. The hml alpha "on" mutations fall into two classes, those that disrupt trans-acting regulators involved in silencing HML alpha and a novel class of mutations that activate HML alpha by insertion at that locus. The hml alpha::Ty "on" mutations illustrate the unusual ability of these retrotransposons to activate genes by overcoming gene silencing mechanisms. The hml alpha::Ty "on" mutations include examples of multimeric Ty arrays. Single Ty and solo delta insertion derivatives of these Ty multimers restore the ability of the silencing mechanism to repress HML alpha.