Open AccessGENETIC ANALYSIS OF DNA REPLICATION IN BACTERIA: dna B MUTATIONS THAT SUPPRESS dnaC MUTATIONS AND dnaQ MUTATIONS THAT SUPPRESS dnaE MUTATIONS IN SALMONELLA TYPHIMURIUM
Author(s) -
Russell Maurer,
Barbara C. Osmond,
David Botstein
Publication year - 1984
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/108.1.25
Subject(s) - biology , genetics , complementation , dna replication , gene , dnab helicase , protein fragment complementation assay , point mutation , mutation , mutant , helicase , rna
We have isolated and characterized extragenic suppressors of mutations in two different target genes that affect DNA replication in Salmonella typhimurium. Both the target and the suppressor genes are functional homologues of known replication genes of E. coli that were identified in intergeneric complementation tests. Our results point to interactions in vivo involving the dnaB and dnaC proteins in one case and the dnaQ and dnaE proteins in the other case. The suppressor mutations, which were isolated as derivatives of lambda-Salmonella in vitro recombinants, were detected by an adaptation of the red plaque complementation assay. This method was applicable even when the locus of suppressor mutations was not chosen in advance.