Open AccessEXCEPTIONALLY HIGH LEVELS OF RESTRICTION SITE POLYMORPHISM IN DNA NEAR THE MAIZE Adh1 GENE
Author(s) -
Mitrick A. Johns,
Judith Strommer,
Michael Freeling
Publication year - 1983
Publication title -
genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.792
H-Index - 246
eISSN - 1943-2631
pISSN - 0016-6731
DOI - 10.1093/genetics/105.3.733
Subject(s) - biology , genetics , allele , restriction site , restriction fragment length polymorphism , restriction enzyme , restriction map , gene , restriction fragment , dna , genotype , nucleic acid sequence
Restriction maps have been prepared for the chromosomal region near seven biochemically and genetically distinct maize alcohol dehydrogenase-1 (Adh1) alleles using a small cDNA probe for Adh1. Five restriction sites spanning about 4 kb in and near the Adh1 transcription unit appear identical in all seven alleles. Outside this conserved region, variation in restriction site position is the rule. Six of the seven alleles are distinguishable, and the alleles appear to fall into four groups. The DNA flanking the 1S-type alleles seems to share no restriction site homology with the DNA near the 1F-type alleles. Several hypotheses are put forward to explain how such high levels of polymorphism could have arisen in a species that has been domesticated for only about 10,000 years.