Open AccessCritical parameters and procedures for anaerobic cultivation of yeasts in bioreactors and anaerobic chambers
Author(s) -
Christiaan Mooiman,
Jonna Bouwknegt,
Wijb J C Dekker,
Sanne J Wiersma,
Raúl A. OrtizMerino,
Erik de Hulster,
Jack T. Pronk
Publication year - 2021
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1093/femsyr/foab035
Subject(s) - anaerobic exercise , bioreactor , biology , yeast , oxygen , microbiology and biotechnology , food science , biochemical engineering , biochemistry , botany , chemistry , physiology , organic chemistry , engineering
All known facultatively fermentative yeasts require molecular oxygen for growth. Only in a small number of yeast species, these requirements can be circumvented by supplementation of known anaerobic growth factors such as nicotinate, sterols and unsaturated fatty acids. Biosynthetic oxygen requirements of yeasts are typically small and, unless extensive precautions are taken to minimize inadvertent entry of trace amounts of oxygen, easily go unnoticed in small-scale laboratory cultivation systems. This paper discusses critical points in the design of anaerobic yeast cultivation experiments in anaerobic chambers and laboratory bioreactors. Serial transfer or continuous cultivation to dilute growth factors present in anaerobically pre-grown inocula, systematic inclusion of control strains and minimizing the impact of oxygen diffusion through tubing are identified as key elements in experimental design. Basic protocols are presented for anaerobic-chamber and bioreactor experiments.