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E2F7, a novel E2F featuring DP‐independent repression of a subset of E2F‐regulated genes
Author(s) -
Di Stefano Luisa,
Jensen Michael Rugaard,
Helin Kristian
Publication year - 2003
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/cdg613
Subject(s) - e2f , biology , repressor , promoter , derepression , ectopic expression , genetics , transcription factor , gene , psychological repression , microbiology and biotechnology , gene expression
The E2F family of transcription factors play an essential role in the regulation of cell cycle progression. In a screen for E2F‐regulated genes we identified a novel E2F family member, E2F7. Like the recently identified E2F‐like proteins of Arabidopsis , E2F7 has two DNA binding domains and binds to the E2F DNA binding consensus site independently of DP co‐factors. Consistent with being an E2F target gene, we found that the expression of E2F7 is cell cycle regulated. Ectopic expression of E2F7 results in suppression of E2F target genes and accumulation of cells in G 1 . Furthermore, E2F7 associates with E2F‐regulated promoters in vivo , and this association increases in S phase. Interestingly, however, E2F7 binds only a subset of E2F‐dependent promoters in vivo , and in agreement with this, inhibition of E2F7 expression results in specific derepression of these promoters. Taken together, these data demonstrate that E2F7 is a unique repressor of a subset of E2F target genes whose products are required for cell cycle progression.