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Xenopus Cut5 is essential for a CDK‐dependent process in the initiation of DNA replication
Author(s) -
Hashimoto Yoshitami,
Takisawa Haruhiko
Publication year - 2003
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/cdg238
Subject(s) - biology , origin recognition complex , control of chromosome duplication , replication factor c , dna replication , eukaryotic dna replication , pre replication complex , chromatin , microbiology and biotechnology , cyclin dependent kinase , replication protein a , dna polymerase delta , s phase , dna , genetics , dna binding protein , cell cycle , transcription factor , cell , rna , reverse transcriptase , gene
Fission yeast Cut5/Rad4 and its budding yeast homolog Dpb11 are required for both DNA replication and the S‐phase checkpoint. Here, we have investigated the role of the Xenopus homolog of Cut5 in the initiation of DNA replication using Xenopus egg extracts. Xenopus Cut5, which shows sequence similarity to DmMus101 and HsTopBP1, is essential for DNA replication in the egg extracts. It is required for the chromatin binding of Cdc45 and DNA polymerases, but not for the formation of pre‐replicative complexes or the elongation stage of DNA replication. The chromatin binding of Cut5 consists of two distinct modes. S‐phase cyclin‐dependent kinase (S‐CDK)‐independent binding is sufficient for DNA replication while S‐CDK‐dependent binding is dispensable. Further, S‐CDK acts after the chromatin binding of Cut5 and before the binding of Cdc45. These results demonstrate that the chromatin binding of Cut5 is required for the action of S‐CDK, which in turn triggers the formation of pre‐initiation complexes of DNA replication.