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Arabidopsis TOBAMOVIRUS MULTIPLICATION (TOM) 2 locus encodes a transmembrane protein that interacts with TOM1
Author(s) -
Tsujimoto Yayoi,
Numaga Takuro,
Ohshima Kiyoshi,
Yano Masaaki,
Ohsawa Ryuji,
Goto Derek B.,
Naito Satoshi,
Ishikawa Masayuki
Publication year - 2003
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/cdg034
Subject(s) - biology , tobamovirus , mutant , genetics , microbiology and biotechnology , transmembrane protein , gene , rna , receptor
The tom2‐1 mutation of Arabidopsis thaliana reduces the efficiency of intracellular multiplication of tobamoviruses. The tom2‐1 mutant was derived from fast‐neutron‐irradiated seeds, and the original mutant line also carries ttm1 , a dominant modifier that increases tobamovirus multiplication efficiency in a tobamovirus‐strain‐specific manner in the tom2‐1 genetic background. Here, we show that the tom2‐1 mutation involved a deletion of ∼20 kb in the nuclear genome. The deleted region included two genes named TOM2A and TOM2B that were both associated with the tom2‐1 phenotype, whereas ttm1 corresponded to the translocation of part of the deleted region that included intact TOM2B but not TOM2A. TOM2A encodes a 280 amino acid putative four‐pass transmembrane protein with a C‐terminal farnesylation signal, while TOM2B encodes a 122 amino acid basic protein. The split‐ubiquitin assay demonstrated an interaction of TOM2A both with itself and with TOM1, an integral membrane protein of A.thaliana presumed to be an essential constituent of tobamovirus replication complex. The data presented here suggest that TOM2A is also an integral part of the tobamovirus replication complex.