Premium
Assisted RNP assembly: SMN and PRMT5 complexes cooperate in the formation of spliceosomal UsnRNPs
Author(s) -
Meister Gunter,
Fischer Utz
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/cdf585
Subject(s) - biology , microbiology and biotechnology , spliceosome , in vitro , rna , snrnp , ribonucleoprotein , computational biology , biophysics , biochemistry , rna splicing , gene
Although spliceosomal Sm proteins can assemble spontaneously onto UsnRNA in vitro , this process requires assisting factors in vivo . SMN, the protein involved in spinal muscular atrophy, is part of a complex that contains the Sm proteins and serves as a critical factor for this reaction. Here, we have reconstituted the SMN‐dependent assembly of UsnRNPs in vitro . We demonstrate that the SMN complex is necessary and sufficient for the assembly reaction. The PRMT5 complex, previously implicated in methylation and storage of Sm proteins, interacts with the SMN complex and enhances its activity in an ATP‐dependent manner. These data uncover the SMN–PRMT5 complex as a functional entity that promotes the assisted assembly of spliceosomal UsnRNPs, and potentially other, RNA–protein complexes.