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Phosphorylation of CIITA directs its oligomerization, accumulation and increased activity on MHCII promoters
Author(s) -
Tosi Giovanna,
JabraneFerrat Nabila,
Peterlin B.Matija
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/cdf557
Subject(s) - ciita , biology , phosphorylation , promoter , transactivation , microbiology and biotechnology , transcription (linguistics) , transcription factor , transcriptional regulation , antigen presentation , mhc class ii , major histocompatibility complex , immune system , gene , gene expression , genetics , t cell , linguistics , philosophy
The class II transactivator (CIITA) is the master regulator of major histocompatibility complex class II (MHCII) transcription. Its activity is regulated at the post‐transcriptional level by phosphorylation and oligomerization. This aggregation mapped to and depended on the phosphorylation of residues between positions 253 and 321 in CIITA, which resulted in a dramatic accumulation of the protein and increased expression of MHCII genes in human promonocytic U937 cells, which represent immature antigen‐presenting cells. Thus, the post‐transcriptional modification of CIITA plays an important role in the immune response.