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Pre‐spliceosome formation in S.pombe requires a stable complex of SF1–U2AF 59 –U2AF 23
Author(s) -
Huang Tao,
Vilardell Josep,
Query Charles C.
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/cdf555
Subject(s) - spliceosome , library science , humanities , biology , philosophy , computer science , genetics , rna , rna splicing , gene
We have initiated a biochemical analysis of splicing complexes in extracts from the fission yeast Schizosaccharomyces pombe . Extracts of S.pombe contain high levels of the spliceosome‐like U2/5/6 tri‐snRNP, which dissociates into mono‐snRNPs in the presence of ATP, and supports binding of U2 snRNP to the 3′ end of introns, yielding a weak ATP‐independent E complex and the stable ATP‐dependent complex A. The requirements for S.pombe complex A formation (pre‐mRNA sequence elements, protein splicing factors, SF1/BBP and both subunits of U2AF) are analogous to those of mammalian complex A. The S.pombe SF1/BBP, U2AF 59 and U2AF 23 are tightly associated in a novel complex that is required for complex A formation. This pre‐formed SF1–U2AF 59 –U2AF 23 complex may represent a streamlined mechanism for recognition of the branch site, pyrimidine tract and 3′ splice site at the 3′ end of introns.