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A cell type‐specific allele of the POU gene Oct‐6 reveals Schwann cell autonomous function in nerve development and regeneration
Author(s) -
Ghazvini Merhnaz,
Mandemakers Wim,
Jaegle Martine,
Piirsoo Marko,
Driegen Siska,
Koutsourakis Manousos,
Smit Xsander,
Grosveld Frank,
Meijer Dies
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/cdf475
Subject(s) - erasmus+ , rotterdam study , biology , sociology , art history , history , population , demography , the renaissance
While an important role for the POU domain transcription factor Oct‐6 in the developing peripheral nerve has been well established, studies into its exact role in nerve development and regeneration have been hampered by the high mortality rate of newborn Oct‐6 mutant animals. In this study we have generated a Schwann cell‐specific Oct‐6 allele through deletion of the Schwann cell‐specific enhancer element (SCE) in the Oct‐6 locus. Analysis of mice homozygous for this allele (Δ SCE allele) reveals that rate‐limiting levels of Oct‐6 in Schwann cells are dependent on the SCE and that this element does not contribute to Oct‐6 regulation in other cell types. We demonstrate a Schwann cell autonomous function for Oct‐6 during nerve development as well as in regenerating nerve. Additionally, we show that Krox‐20, an important regulatory target of Oct‐6 in Schwann cells, is activated, with delayed kinetics, through an Oct‐6‐independent mechanism in these mice.