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TIS7 interacts with the mammalian SIN3 histone deacetylase complex in epithelial cells
Author(s) -
Vietor Ilja,
Vadivelu Santhosh K.,
Wick Nikolaus,
Hoffman Robert,
Cotten Matt,
Seiser Christian,
Fialka Irene,
Wunderlich Winfried,
Haase Astrid,
Korinkova Gabriela,
Brosch Gerald,
Huber Lukas A.
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/cdf461
Subject(s) - biology , hdac11 , histone deacetylase , hdac4 , histone deacetylase 2 , histone deacetylase 5 , microbiology and biotechnology , hdac10 , histone , sap30 , computational biology , genetics , gene
The mammalian SIN3 complex consists of histone deacetylases (HDAC1, HDAC2), several known proteins (SAP30, N‐CoR) and as yet unidentified proteins. Here we show that the mouse tetradecanoyl phorbol acetate induced sequence 7 (TIS7) protein is a novel transcriptional co‐repressor that can associate with the SIN3 complex. We have identified tis7 as a gene that is up‐regulated upon loss of polarity in a mouse mammary gland epithelial cell line expressing an estrogen‐inducible c‐JunER fusion protein. In unpolarized cells, TIS7 protein levels increase and TIS7 translocates into the nucleus. Overexpression of tis7 causes loss of polarity and represses a set of genes, as revealed by cDNA microarray analysis. We have shown that TIS7 protein interacts with several proteins of the SIN3 complex (mSin3B, HDAC1, N‐CoR and SAP30) by yeast two‐hybrid screening and co‐immunoprecipitations. TIS7 co‐immunoprecipitated HDAC complex is enzymatically active and represses a GAL4‐dependent reporter transcription. The transcriptional repression of endogenous genes by tis7 overexpression is HDAC dependent. Thus, we propose TIS7 as a transcriptional co‐repressor affecting the expression of specific genes in a HDAC activity‐dependent manner during cell fate decisions, e.g. scattering.