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A satellite phage‐encoded antirepressor induces repressor aggregation and cholera toxin gene transfer
Author(s) -
Davis Brigid M.,
Kimsey Harvey H.,
Kane Anne V.,
Waldor Matthew K.
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/cdf427
Subject(s) - prophage , vibrio cholerae , biology , repressor , bacteriophage , cholera toxin , el tor , genetics , gene , virulence , filamentous bacteriophage , microbiology and biotechnology , escherichia coli , transcription factor , bacteria
CTXφ is a filamentous bacteriophage whose genome encodes cholera toxin, the principal virulence factor of Vibrio cholerae . We have found that the CTXφ‐related element RS1 is a satellite phage whose transmission depends upon proteins produced from a CTX prophage (its helper phage). However, unlike other satellite phages and satellite animal viruses, RS1 can aid the CTX prophage as well as exploit it, due to the RS1‐encoded protein RstC. RstC, whose function previously was unknown, is an antirepressor that counteracts the activity of the phage repressor RstR. RstC promotes transcription of genes required for phage production and thereby promotes transmission of both RS1 and CTXφ. Antirepression by RstC also induces expression of the cholera toxin genes, ctxAB , and thus may contribute to the virulence of V.cholerae . In vitro , RstC binds directly to RstR, producing unusual, insoluble aggregates containing both proteins. In vivo , RstC and RstR are both found at the cell pole, where they again appear to form stable complexes. The sequestration/inactivation process induced by RstC resembles those induced by mutant polyglutamine‐containing proteins implicated in human neurodegenerative disorders.