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Deconstructing PML‐induced premature senescence
Author(s) -
Bischof Oliver,
Kirsh Olivier,
Pearson Mark,
Itahana Koji,
Pelicci Pier Giuseppe,
Dejean Anne
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/cdf341
Subject(s) - biology , senescence , promyelocytic leukemia protein , gene isoform , telomerase , microbiology and biotechnology , telomere , phosphorylation , nuclear protein , genetics , transcription factor , dna , gene
In this study, we investigated the subcellular and molecular mechanisms underlying promyelocytic leu kemia (PML)‐induced premature senescence. We demonstrate that intact PML nuclear bodies are not required for the induction of senescence. We have determined further that of seven known PML isoforms, only PML IV is capable of causing premature senescence, providing the first evidence for functional differences among these isoforms. Of interest is the fact that in contrast to PML +/+ fibroblasts, PML −/− cells are resistant to PML IV‐induced senescence. This suggests that although PML IV is necessary for this process to occur, it is not sufficient and requires other components for activity. Finally, we provide evidence that PML IV‐induced senescence involves stabilization and activation of p53 through phosphorylation at Ser46 and acetylation at Lys382, and that it occurs independently of telomerase and differs from that elicited by oncogenic Ras. Taken together, our data assign a specific pro‐senescent activity to an individual PML isoform that involves p53 activation and is independent from PML nuclear bodies.