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Differential mRNA translation and meiotic progression require Cdc2‐mediated CPEB destruction
Author(s) -
Mendez Raul,
Barnard Daron,
Richter Joel D.
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/21.7.1833
Subject(s) - polyadenylation , biology , mitosis , microbiology and biotechnology , xenopus , metaphase , translation (biology) , messenger rna , genetics , gene , chromosome
Translational activation of several dormant mRNAs in vertebrate oocytes is mediated by cytoplasmic polyadenylation, a process controlled by the cytoplasmic polyadenylation element (CPE) and its binding protein CPEB. The translation of CPE‐containing mRNAs does not occur en masse at any one time, but instead is temporally regulated. We show here that in Xenopus , partial destruction of CPEB controls the temporal translation of CPE‐containing mRNAs. While some mRNAs, such as the one encoding Mos, are polyadenylated at prophase I, the polyadenylation of cyclin B1 mRNA requires the partial destruction of CPEB that occurs at metaphase I. CPEB destruction is mediated by a PEST box and Cdc2‐catalyzed phosphorylation, and is essential for meiotic progression to metaphase II. CPEB destruction is also necessary for mitosis in the early embryo. These data indicate that a change in the CPEB:CPE ratio is necessary to activate mRNAs at metaphase I and drive the cells' entry into metaphase II.