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Human Mps1 kinase is required for the spindle assembly checkpoint but not for centrosome duplication
Author(s) -
Stucke Volker M.,
Silljé Herman H. W.,
Arnaud Lionel,
Nigg Erich A.
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/21.7.1723
Subject(s) - biology , centrosome , gene duplication , microbiology and biotechnology , spindle checkpoint , checkpoint kinase 2 , genetics , protein serine threonine kinases , chek1 , cell cycle protein , kinase , computational biology , kinetochore , cell cycle checkpoint , protein kinase a , cell cycle , gene , chromosome
Budding yeast Mps1p kinase has been implicated in both the duplication of microtubule‐organizing centers and the spindle assembly checkpoint. Here we show that hMps1, the human homolog of yeast Mps1p, is a cell cycle‐regulated kinase with maximal activity during M phase. hMps1 localizes to kinetochores and its activity and phosphorylation state increase upon activation of the mitotic checkpoint. By antibody microinjection and siRNA, we demonstrate that hMps1 is required for human cells to undergo checkpoint arrest in response to microtubule depolymerization. In contrast, centrosome (re‐)duplication as well as cell division occur in the absence of hMps1. We conclude that hMps1 is required for the spindle assembly checkpoint but not for centrosome duplication.