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Regulation of intracellular trafficking of human CD1d by association with MHC class II molecules
Author(s) -
Kang SukJo,
Cresswell Peter
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/21.7.1650
Subject(s) - biology , endosome , microbiology and biotechnology , cd1d , cd74 , mhc class i , endoplasmic reticulum , mhc class ii , major histocompatibility complex , intracellular , transfection , cd1 , antigen , biochemistry , antigen presenting cell , genetics , cd8 , in vitro , gene , cytotoxic t cell , natural killer t cell
CD1 family members are antigen‐presenting molecules capable of presenting bacterial or synthetic glycolipids to T cells. Here we show that a subset of human CD1d molecules are associated with major histocompatibility complex (MHC) class II molecules, both on the cell surface and in the late endosomal/lysosomal compartments where class II molecules transiently accumulate during transport. The interaction is initiated in the endoplasmic reticulum with class II–invariant chain complexes and appears to be maintained throughout the class II trafficking pathway. A truncated form of CD1d which lacks its cytoplasmic YXXZ internalization motif is transported to late endosomal/lysosomal compartments in the presence of class II molecules. Furthermore, the same CD1d deletion mutant is targeted to lysosomal compartments in HeLa cells expressing class II molecules and invariant chain by transfection. The deletion mutant was also found in lysosomal compartments in HeLa cells expressing only the p33 form of the invariant chain. These data suggest that the intracellular trafficking pathway of CD1d may be altered by class II molecules and invariant chain induced during inflammation.