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Intracellular trafficking pathway of newly synthesized CD1b molecules
Author(s) -
Briken V.,
Jackman R.M.,
Dasgupta S.,
Hoening S.,
Porcelli S.A.
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/21.4.825
Subject(s) - biology , microbiology and biotechnology , endocytic cycle , antigen presentation , antigen processing , endosome , major histocompatibility complex , mhc class i , antigen , intracellular , internalization , golgi apparatus , t cell , endocytosis , biochemistry , cell , endoplasmic reticulum , immune system , immunology
The intracellular trafficking of major histocompatibility complex (MHC) class I and class II molecules has evolved to support their function in peptide antigen presentation optimally. We have analyzed the intracellular trafficking of newly synthesized human CD1b, a lipid antigen‐presenting molecule, to understand how this relates to its antigen‐presenting function. Nascent CD1b was transported rapidly to the cell surface after leaving the Golgi, and then entered the endocytic system by internalization via AP‐2‐dependent sorting at the plasma membrane. A second sorting event, possibly involving AP‐3 complexes, led to prominent accumulation of CD1b in MHC class II compartments (MIICs). Functional studies demonstrated the importance of nascent CD1b for the efficient presentation of a foreign lipid antigen. Therefore, the intracellular trafficking of nascent CD1b via the cell surface to reach MIICs may allow the efficient sampling of lipid antigens present in endocytic compartments.