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Four new subunits of the Dam1–Duo1 complex reveal novel functions in sister kinetochore biorientation
Author(s) -
Janke Carsten,
Ortíz Jennifer,
Tanaka Tomoyuki U.,
Lechner Johannes,
Schiebel Elmar
Publication year - 2002
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/21.1.181
Subject(s) - library science , computer science , sister , art history , history , political science , law
We show here that Ask1p, Dad2p, Spc19p and Spc34p are subunits of the budding yeast Duo1p–Dam1p–Dad1p complex, which associate with kinetochores and localize along metaphase and anaphase spindles. Analysis of spc34‐3 cells revealed three novel functions of the Duo1–Dam1p–Dad1p subunit Spc34p. First, SPC34 is required to establish biorientation of sister kinetochores. Secondly, SPC34 is essential to maintain biorientation. Thirdly, SPC34 is necessary to maintain an anaphase spindle independently of chromosome segregation. Moreover, we show that in spc34‐3 cells, sister centromeres preferentially associate with the pre‐existing, old spindle pole body (SPB). A similar preferential attachment of sister centromeres to the old SPB occurs in cells depleted of the cohesin Scc1p, a protein with a known role in facilitating biorientation. Thus, the two SPBs are not equally active in early S phase. We suggest that not only in spc34‐3 and Δscc1 cells but also in wild‐type cells, sister centromeres bind after replication preferentially to microtubules organized by the old SPB. Monopolar attached sister centromeres are resolved to bipolar attachment in wild‐type cells but persist in spc34‐3 cells.