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cdc42 regulates the exit of apical and basolateral proteins from the trans ‐Golgi network
Author(s) -
Müsch Anne,
Cohen David,
Kreitzer Geri,
RodriguezBoulan Enrique
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.9.2171
Subject(s) - microbiology and biotechnology , biology , golgi apparatus , cdc42 , actin cytoskeleton , actin remodeling , cytoskeleton , gtpase , epithelial polarity , actin , mdia1 , vesicle , cell polarity , actin remodeling of neurons , biochemistry , cell , endoplasmic reticulum , membrane
It is well established that Rho‐GTPases regulate vesicle fusion and fission events at the plasma membrane through their modulatory role on the cortical actin cytoskeleton. In contrast, their effects on intracellular transport processes and actin pools are less clear. It was recently shown that cdc42 associates with the Golgi apparatus in an ARF‐dependent manner, similarly to coat proteins involved in vesicle formation and to several actin‐binding proteins. We report here that mutants of cdc42 inhibited the exit of basolateral proteins from the trans ‐Golgi network (TGN), while stimulating the exit of an apical marker, in two different transport assays. This regulation may result from modulation of the actin cytoskeleton, as GTPase‐deficient cdc42 depleted a perinuclear actin pool that rapidly exchanges with exogenous fluorescent actin.