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Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae
Author(s) -
Kamimura Yoichiro,
Tak YonSoo,
Sugino Akio,
Araki Hiroyuki
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.8.2097
Subject(s) - biology , origin recognition complex , control of chromosome duplication , pre replication complex , replication factor c , dna replication , eukaryotic dna replication , dna replication factor cdt1 , genetics , origin of replication , minichromosome maintenance , seqa protein domain , licensing factor , microbiology and biotechnology , dna
Cdc45, which binds to the minichromosomal maintenance (Mcm) proteins, has a pivotal role in the initiation and elongation steps of chromosomal DNA replication in eukaryotes. Here we show that throughout the cell cycle in Saccharomyces cerevisiae , Cdc45 forms a complex with a novel factor, Sld3. Consistently, Sld3 and Cdc45 associate simultaneously with replication origins in the chromatin immunoprecipitation assay: both proteins associate with early‐firing origins in G 1 phase and with late‐firing origins in late S phase. Moreover, the origin associations of Sld3 and Cdc45 are mutually dependent. The temperature‐sensitive sld3 mutation confers a defect in DNA replication at the restrictive temperature and reduces an interaction not only between Sld3 and Cdc45, but also between Cdc45 and Mcm2. These results suggest that the Sld3‐Cdc45 complex associates with replication origins through Mcm proteins. At the restrictive temperature in sld3‐5 cells, replication factor A, a single‐strand DNA binding protein, does not associate with origins. Therefore, the origin association of Sld3‐Cdc45 complex is prerequisite for origin unwinding in the initiation of DNA replication.