Premium
The Arabidopsis thaliana ABC transporter At MRP5 controls root development and stomata movement
Author(s) -
Gaedeke Nicola,
Klein Markus,
Kolukisaoglu Uener,
Forestier Cyrille,
Müller Axel,
Ansorge Mark,
Becker Dirk,
Mamnun Yasmine,
Kuchler Karl,
Schulz Burkhard,
MuellerRoeber Bernd,
Martinoia Enrico
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.8.1875
Subject(s) - arabidopsis thaliana , guard cell , auxin , arabidopsis , mutant , vascular bundle , biology , transporter , root hair , microbiology and biotechnology , epidermis (zoology) , wild type , phloem , botany , biochemistry , gene , anatomy
In the present study, we investigated a new member of the ABC transporter superfamily of Arabidopsis thaliana , At MRP5. AtMRP5 encodes a 167 kDa protein and exhibits low glutathione conjugate and glucuronide conjugate transport activity. Promotor‐β‐glucuronidase fusion constructs showed that AtMRP5 is expressed mainly in the vascular bundle and in the epidermis, especially guard cells. Using reverse genetics, we identified a plant with a T‐DNA insertion in AtMRP5 (mrp5‐1). mrp5‐1 exhibited decreased root growth and increased lateral root formation. Auxin levels in the roots of mrp5‐1 plants were increased. This observation may indicate that At MRP5 works as an auxin conjugate transporter or that mutant plants are affected in ion uptake, which may lead to changes in auxin concentrations. Experiments on epidermal strips showed that in contrast to wild type, the sulfonylurea glibenclamide had no effect on stomatal opening in mrp5‐1 plants. This result strongly suggests that At MRP5 may also function as an ion channel regulator.