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Perinuclear, perigranular and sub‐plasmalemmal mitochondria have distinct functions in the regulation of cellular calcium transport
Author(s) -
Park Myoung Kyu,
Ashby Michael C.,
Erdemli Gul,
Petersen Ole H.,
Tepikin Alexei V.
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.8.1863
Subject(s) - biology , mitochondrion , microbiology and biotechnology , calcium , organic chemistry , chemistry
We have identified three distinct groups of mitochondria in normal living pancreatic acinar cells, located (i) in the peripheral basolateral region close to the plasma membrane, (ii) around the nucleus and (iii) in the periphery of the granular region separating the granules from the basolateral area. Three‐dimensional reconstruction of confocal slices showed that the perigranular mitochondria form a barrier surrounding the whole of the granular region. Cytosolic Ca 2+ oscillations initiated in the granular area triggered mitochondrial Ca 2+ uptake mainly in the perigranular area. The most intensive uptake occurred in the mitochondria close to the apical plasma membrane. Store‐operated Ca 2+ influx through the basolateral membrane caused preferential Ca 2+ uptake into sub‐plasmalemmal mitochondria. The perinuclear mitochondria were activated specifically by local uncaging of Ca 2+ in the nucleus. These mitochondria could isolate nuclear and cytosolic Ca 2+ signalling. Photobleaching experiments indicated that different groups of mitochondria were not luminally connected. The three mitochondrial groups are activated independently by specific spatiotemporal patterns of cytosolic Ca 2+ signals and can therefore participate in the local regulation of Ca 2+ homeostasis and energy supply.