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The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control
Author(s) -
Janke Carsten,
Ortiz Jennifer,
Lechner Johannes,
Shevchenko Anna,
Shevchenko Andrej,
Magiera Maria M.,
Schramm Carolin,
Schiebel Elmar
Publication year - 2001
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1093/emboj/20.4.777
Subject(s) - kinetochore , biology , centromere , microbiology and biotechnology , nocodazole , spindle checkpoint , mitosis , chromosome segregation , mitotic exit , g2 m dna damage checkpoint , genetics , spindle apparatus , cell cycle checkpoint , chromosome , cell cycle , cell division , cell , cytoskeleton , gene
Here, we show that the budding yeast proteins Ndc80p, Nuf2p, Spc24p and Spc25p interact at the kinetochore. Consistently, Ndc80p, Nuf2p, Spc24p and Spc25p associate with centromere DNA in chromatin immunoprecipitation experiments, and SPC24 interacts genetically with MCM21 encoding a kinetochore component. Moreover, although conditional lethal spc24‐2 and spc25‐7 cells form a mitotic spindle, the kinetochores remain in the mother cell body and fail to segregate the chromosomes. Despite this defect in chromosome segregation, spc24‐2 and spc25‐7 cells do not arrest in metaphase in response to checkpoint control. Furthermore, spc24‐2 cells showed a mitotic checkpoint defect when microtubules were depolymerized with nocodazole, indicating that Spc24p has a function in checkpoint control. Since Ndc80p, Nuf2p and Spc24p are conserved proteins, it is likely that similar complexes are part of the kinetochore in other organisms.